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Image Search Results


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Journal: Neuron

Article Title: The autism-associated gene Scn2a contributes to dendritic excitability and synaptic function in prefrontal cortex

doi: 10.1016/j.neuron.2019.05.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains AAV5-Ef1α-Cre-mCherry UNC Viral Core N/A Chemicals, Peptides, and Recombinant Proteins NBQX disodium salt Tocris 0373 Tetrodotoxin-citrate Alomone T-550 ( R )-CPP Tocris 0247 Picrotoxin Tocris 1128 Fluo-5F, Pentapotassium Salt, cell impermeant Invitrogen F14221 Alexa Fluor 594 hydrazide Invitrogen A10438 Prolong Gold Antifade with DAPI Life Technologies P36941 Experimental Models: Organisms/Strains Mouse: Ai14: 6;129S6- Gt(ROSA)26Sor tm14(CAG-tdTomato)Hze /J The Jackson Laboratory JAX: 007908 Mouse: C57B6J The Jackson Laboratory JAX: 000664 Mouse: B6.Cg-Tg(Camk2a-cre)T29–1Stl/J The Jackson Laboratory JAX:005359 Mouse: Scn2a +/− M. Montal / E. Glasscock PMID: 10827969, 28334922 Mouse: Scn2a +/fl This study N/A Software and Algorithms IGOR Pro Wavemetrics RRID:SCR_000325; v6.3 FIJI https://fiji.sc/ RRID:SCR_002285 MATLAB Mathworks RRID:SCR_001622; v2016a Python pythn.org RRID:SCR_008394; v3.5.2 IMARIS Bitplane RRID:SCR_007370; v6.3 Prism Graphpad RRID:SCR_002798; v8 Statview SAS v5.0.1 Ethovision Noldus RRID:SCR_000441 Open in a separate window KEY RESOURCES TABLE Protein truncation in SCN2A /Na V 1.2 bears considerable autism spectrum disorder risk Na V 1.2 governs action potential initiation in immature neocortical pyramidal cells Mature Scn2a +/− neurons have impaired dendritic excitability and excitatory synapses Cell-autonomous loss of Na V 1.2 in mature cells alone impairs synaptic function

Techniques: Virus, Recombinant, Software